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NWSPEC

UHPLC System for Pharma, Pesticide & Protein Analysis | 16,000 psi | LC80

UHPLC System for Pharma, Pesticide & Protein Analysis | 16,000 psi | LC80

UHPLC System Order Guide

Ultra-High Performance Liquid Chromatography for Global Laboratories

System Overview

Next-generation UHPLC system with unified 16,000 psi (105 MPa / 1,050 bar) pressure capability across pump and autosampler. Features ≤150 μL system delay volume, ≤0.0005% carryover, and validated 0.039% RSD retention time reproducibility. Engineered for trace-level analysis of pesticides, veterinary residues, pharmaceutical impurities, antibiotics, and proteins in complex matrices.

Unified Pressure Architecture: Pump 16,000 psi | Autosampler 16,000 psi | Delay Volume ≤150 μL | Flow Precision ≤0.06% RSD | Carryover ≤0.0005%

Step 1: Select Pump Configuration

Standard UHPLC Pump

4-Channel Pressure Feedback | Auto-Priming | Compressibility Compensation
Max Pressure: 16,000 psi (105 MPa / 1,050 bar)
Flow Range: 0.001–5.00 mL/min
Delay Volume: ≤150 μL
Gradient: Binary High-Pressure Mixing (A1/A2, B1/B2)
Precision: ≤0.06% RSD
Degasser: 2-Channel Integrated Vacuum
Select Standard

Step 2: Select Autosampler

Standard Autosampler

Needle-Loading Non-Destructive Injection | 162-Sample Capacity | Visual Window
Sample Capacity: ≥162 × 2 mL vials or 3 × 96-well microplates
Injection Mode: Needle-Loading (Zero Sample Loss)
Injection Range: 0.1–30 μL (Expandable)
Precision: ≤0.25% RSD
Max Pressure: 16,000 psi (105 MPa / 1,050 bar)
Carryover: ≤0.0005% (Caffeine, UV)
Operation: Touch-Button Auto Door, Auto-Eject Tray
Select Standard

Step 3: Select Column Oven

Step 4: Select Detector(s)

Detector Specifications Key Features Select
Photodiode Array (PDA)
Full-Spectrum Qualitative & Quantitative
Wavelength: 190-800 nm
Light Source: Single Deuterium Lamp
Sampling: ≥80 Hz
Noise: ≤2×10⁻⁵ AU
Single-Lamp Full Coverage
10-Step Digital Resolution
Refractive Index (RID)
Universal Non-Chromophore Detection
Range: 1.00-1.75 RI
Linearity: 600 μRIU
Sampling: >85 Hz
Noise: ≤2.5 nRIU
3-Zone Independent Control
Low Power Mode
Overtemp + Leak Protection
Fluorescence (FLD)
Trace-Level Fluorescent Compounds
Ex: 200-750 nm
Em: 210-750 nm
Sampling: ≥115 Hz
S/N: ≥1500 (Water Raman)
4-Channel Simultaneous
3D Fluorescence Scanning
Filter Auto-Calibration
PMT Normalization
Evaporative Light Scattering (ELSD)
Non-UV, Non-Volatile Compounds
Evaporation: RT-150°C
Nebulization: 35-60°C
Sampling: ≥95 Hz
LOD: ≤0.5 ng (Cholesterol)
650nm Laser Optional
High-Sensitivity PMT
1-6 Sensitivity Levels
Low Power Protection

Step 5: Software & Compliance Options

Chromatography Software Suite 21 CFR Part 11

Database management | Multi-language interface (EN/DE/FR/ES/RU) | Audit trail | Real-time equipment monitoring | Automatic delay volume compensation | Consumables lifetime management

Seal Wash Kit

Included with Advanced pump configuration. Extends plunger rod lifetime with high-salt mobile phases

Intelligent Weighing Tray

Mobile phase consumption monitoring with precision alerts (included with Advanced pump)

Column Switching Valve Kit

2-position valves enabling multi-dimensional LC and method development screening

IQ/OQ/PQ Validation Documentation GMP/GLP

Installation, operational, and performance qualification protocols for regulated environments

System Highlights

  • Unified 16,000 psi Pressure Architecture: Both pump and autosampler rated to 16,000 psi (105 MPa) for seamless sub-2μm separations
  • Minimal Delay Volume: ≤150 μL system volume for rapid gradient response
  • Non-Destructive Injection: Needle-loading technology with zero sample loss
  • Superior Precision: ≤0.06% RSD flow, ≤0.25% RSD injection, 0.039% RSD retention time
  • Zero Carryover: ≤0.0005% (caffeine, UV detection)
  • Intelligent Control: Auto-priming, real-time compressibility compensation, delay volume auto-compensation
  • Global Compliance: 21 CFR Part 11, GMP/GLP ready, multi-language software
  • Biological Sample Protection: Cooled autosampler maintains sample integrity

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Configure your UHPLC system with unified 16,000 psi pressure architecture and receive comprehensive pricing within 24 hours

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Since 1993 30+ Years of Instrument Expertise
Reliable Manufacturer Manufacturing & Services
Ph.D. Founded Dr. Pingxin Wang, UT Austin
Advantages Price · Service · Speed · Technical · Data
Fast Shipping In-Stock Orders Ship Same Day

Frequently Asked Questions

How can I contact you?

Feel free to call us T+86 189-6516-4839
or email  sales@nwspec.com to:

• Place an order
• Ask for a quote
• Or if you have any other questions

You will always get a live person here at NWSPEC; never an automated system! 

We are open from 6:00AM - 7:00PM Eastern Time, Monday - Friday. 

Where can I find application notes?

If you have any questions regarding the application notes, or can't find the one you are looking for, please contact Sales at sales@nwspec.com



Do you have instructional manuals for your instruments to download?

Yes, we have instructional manuals for our instruments. They can be found on https://nwspec.com/pages/resource .

If you don't see the one you are looking for, please contact: sales@nwspec.com



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